Hotspot

psaI-ycf4

LSC · 60430-60841 · 412 bp

Back to Bulbophyllum

Pi 0.0325

MarkerSeek score 77.90

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 412
variable_sites: 62
pi: 0.0325
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATCGCAATGGCTTCTTT	TTGTTTTTCGAGACCCCGTT	45.0	45.0	58.0	58.0	377-539 bp	0.947368	88.9
psaI-ycf4_p2	GCAATCGCAATGGCTTCTTT	CTTGTTTTTCGAGACCCCGT	45.0	50.0	58.0	58.4	378-540 bp	0.947368	85.9
psaI-ycf4_p3	CTAGTTTTTCCGGCAATCGC	TTGTTTTTCGAGACCCCGTT	50.0	45.0	57.8	58.0	389-551 bp	0.894737	84.9
psaI-ycf4_p4	GTTTTTCCGGCAATCGCAAT	TTGTTTTTCGAGACCCCGTT	45.0	45.0	58.3	58.0	386-548 bp	0.894737	84.0
psaI-ycf4_p5	TTTTTCCGGCAATCGCAATG	TTGTTTTTCGAGACCCCGTT	45.0	45.0	58.3	58.0	385-547 bp	0.894737	84.0