Hotspot

accD-psaI

LSC · 59483-60318 · 836 bp

Back to Bulbophyllum

Pi 0.0379

MarkerSeek score 79.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 836
variable_sites: 147
pi: 0.0379
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCTACAACTGGCGGAGTTAC	AAAGAAGCCATTGCGATTGC	55.0	45.0	58.0	58.0	1082-1195 bp	1.0	91.9
accD-psaI_p2	ACCCGTTTGATCGAGTATGC	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.1	58.0	1259-1372 bp	1.0	91.5
accD-psaI_p3	CCTACAACTGGCGGAGTTAC	TTGCTGAGACGGAAATACGG	55.0	50.0	58.0	58.0	1594-1809 bp	0.947368	89.8
accD-psaI_p4	CCTACAACTGGCGGAGTTAC	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.0	58.0	1484-1699 bp	0.947368	89.5
accD-psaI_p5	CCTACAACTGGCGGAGTTAC	TCAACTAGCAGCATTCCGAC	55.0	50.0	58.0	58.0	1418-1543 bp	0.789474	83.5