Hotspot

atpB-rbcL

LSC · 54733-55754 · 1022 bp

Back to Bulbophyllum

Pi 0.0347

MarkerSeek score 77.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1022
variable_sites: 194
pi: 0.0347
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AGCCACTTTGGATTCTGCAA	AGAAACGATCTCTCCAACGC	45.0	50.0	58.0	58.0	2344-2468 bp	1.0	92.0
atpB-rbcL_p2	AGCCACTTTGGATTCTGCAA	GTCCACACAGTTGTCCATGT	45.0	50.0	58.0	58.0	1901-2025 bp	1.0	91.7
atpB-rbcL_p3	GAGGAGTTCCCGTGTCAATC	AGAAACGATCTCTCCAACGC	55.0	50.0	58.0	58.0	1937-2061 bp	0.947368	89.8
atpB-rbcL_p4	TTTTTCCTCCACGGCGATAG	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2147-2271 bp	0.947368	87.9
atpB-rbcL_p5	AGGGGGCTAAAAGATCCACT	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2129-2253 bp	0.894737	85.6