Hotspot

psaA-ycf3

LSC · 42865-43419 · 555 bp

Back to Bulbophyllum

Pi 0.0307

MarkerSeek score 81.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 555
variable_sites: 87
pi: 0.0307
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	50.0	50.0	57.9	58.2	699-741 bp	1.0	89.6
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	698-740 bp	1.0	89.6
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	GTTCGATCAAGCTGCTGAGT	50.0	50.0	57.9	58.3	733-775 bp	1.0	89.0
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	702-744 bp	1.0	88.4
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	CTTGGTTCGATCAAGCTGCT	50.0	50.0	57.9	58.6	737-779 bp	1.0	86.8