Hotspot

psbM-trnD-GUC

LSC · 30300-31380 · 1081 bp

Back to Bulbophyllum

Pi 0.0408

MarkerSeek score 80.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1081
variable_sites: 208
pi: 0.0408
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CGCAAGAATATTGACTTCCA	CCATGCATATGGCTGGGATT	40.0	50.0	53.7	58.1	999-1240 bp	1.0	57.2
psbM-trnD-GUC_p2	CGCAAGAATATTGACTTCCA	AGTTCAATCGGTCAGAGCAC	40.0	50.0	53.7	57.9	977-1218 bp	1.0	57.0
psbM-trnD-GUC_p3	CGCAAGAATATTGACTTCCAT	CCATGCATATGGCTGGGATT	38.1	50.0	54.4	58.1	999-1240 bp	1.0	54.3
psbM-trnD-GUC_p4	CGCAAGAATATTGACTTCCAT	AGTTCAATCGGTCAGAGCAC	38.1	50.0	54.4	57.9	977-1218 bp	1.0	54.1
psbM-trnD-GUC_p5	CGCAAGAATATTGACTTCCA	ATGCATATGGCTGGGATTGT	40.0	45.0	53.7	57.3	997-1238 bp	1.0	52.2