Hotspot

trnC-GCA-petN

LSC · 28561-29348 · 788 bp

Back to Bulbophyllum

Pi 0.0253

MarkerSeek score 79.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 115
pi: 0.0253
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CCAGTTCGAATCTGGGTGTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.3	58.2	782-849 bp	1.0	88.6
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.5	58.2	805-872 bp	1.0	86.6
trnC-GCA-petN_p3	CAGTTCGAATCTGGGTGTCG	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.6	58.2	781-848 bp	1.0	85.6
trnC-GCA-petN_p4	TTTTGGATGGAATTTGGCGG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.2	58.2	844-911 bp	1.0	84.7
trnC-GCA-petN_p5	CCAGTTCGAATCTGGGTGTC	GCCCAAGCGAGACTTACTAT	55.0	50.0	58.3	57.1	781-848 bp	1.0	82.7