Hotspot

trnS-GCU-trnG-UCC

LSC · 8458-9378 · 921 bp

Back to Bulbophyllum

Pi 0.0574

MarkerSeek score 85.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 921
variable_sites: 245
pi: 0.0574
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	928-1157 bp	1.0	60.4
trnS-GCU-trnG-UCC_p2	AGTCCACTCAGCCATCTCTC	ACGAATCACACTTTTACCACT	55.0	38.1	58.5	55.5	923-1152 bp	1.0	59.6
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	AGAACGAATCACACTTTTACCAC	50.0	39.1	57.6	57.3	931-1160 bp	1.0	59.4
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	GAACGAATCACACTTTTACCACT	50.0	39.1	57.6	57.3	930-1159 bp	1.0	59.4
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	GAACGAATCACACTTTTACCACT	55.0	39.1	58.5	57.3	925-1154 bp	1.0	58.5