Hotspot

psbI-trnS-GCU

LSC · 8198-8369 · 172 bp

Back to Bulbophyllum

Pi 0.0502

MarkerSeek score 78.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 45
pi: 0.0502
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	818-892 bp	1.0	91.5
psbI-trnS-GCU_p2	ATCTAATGATCCGGGGCGTA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	272-285 bp	1.0	91.4
psbI-trnS-GCU_p3	TATCTAATGATCCGGGGCGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	273-286 bp	1.0	91.4
psbI-trnS-GCU_p4	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	839-893 bp	0.842105	85.2
psbI-trnS-GCU_p5	ATCTAATGATCCGGGGCGTA	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	278-286 bp	0.842105	84.5