Hotspot

psbK-psbI

LSC · 7589-8086 · 498 bp

Back to Bulbophyllum

Pi 0.0378

MarkerSeek score 77.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 498
variable_sites: 94
pi: 0.0378
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	565-629 bp	1.0	91.0
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	566-630 bp	1.0	91.0
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTACGCCCCGGATCATTAGA	50.0	50.0	57.9	58.3	567-631 bp	1.0	89.2
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.3	58.1	562-626 bp	1.0	89.0
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.3	58.1	563-627 bp	1.0	89.0