Hotspot

ndhF

SSC · 110611-112866 · 2256 bp

Back to Buddleja

Pi 0.00749

MarkerSeek score 46.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2256
variable_sites: 115
pi: 0.00749
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGGTGGAAAGTGAGGAAGA	GCCCAATACCCCTTCCTTTT	50.0	50.0	57.9	57.8	2939-2975 bp	0.5	59.4
ndhF_p2	CGGAAGAAAGGGTGGAAAGT	GCCCAATACCCCTTCCTTTT	50.0	50.0	57.7	57.8	2947-2983 bp	0.5	58.2
ndhF_p3	AGGGTGGAAAGTGAGGAAGA	TCCCCAAGGAAAACGCTTTT	50.0	45.0	57.9	58.2	2965-2995 bp	0.464286	58.1
ndhF_p4	ACGGAAGAAAGGGTGGAAAG	TCCCCAAGGAAAACGCTTTT	50.0	45.0	57.7	58.2	2974-2987 bp	0.428571	55.4
ndhF_p5	CGGAAGAAAGGGTGGAAAGT	TCCCCAAGGAAAACGCTTTT	50.0	45.0	57.7	58.2	2973-2986 bp	0.428571	55.4