Hotspot

ndhD-psaC

SSC · 117338-117461 · 124 bp

Back to Buddleja

Pi 0.035

MarkerSeek score 64.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 124
variable_sites: 15
pi: 0.035
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAACCCGCAAATATTGGCAA	CCGACGGATTTCTTGAGTGT	45.0	50.0	57.3	57.9	252-263 bp	1.0	82.8
ndhD-psaC_p2	GAACCCGCAAATATTGGCAA	ATGGTCTGAAACAACTCGCA	45.0	45.0	57.3	57.7	220-231 bp	1.0	82.4
ndhD-psaC_p3	AGGAACCCGCAAATATTGGC	CCGACGGATTTCTTGAGTGT	50.0	50.0	58.9	57.9	254-265 bp	1.0	81.0
ndhD-psaC_p4	GAACCCGCAAATATTGGCAA	TCCGACGGATTTCTTGAGTG	45.0	50.0	57.3	57.6	253-264 bp	1.0	80.5
ndhD-psaC_p5	GAACCCGCAAATATTGGCAA	AAGAGATGTGAATCCGCCTG	45.0	50.0	57.3	57.7	273-284 bp	1.0	80.2