Hotspot

rps16-trnQ-UUG

LSC · 6211-7326 · 1116 bp

Back to Buddleja

Pi 0.0163

MarkerSeek score 73.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 107
pi: 0.0163
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1161-1222 bp	1.0	89.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1219-1280 bp	0.964286	88.0
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1175-1236 bp	1.0	87.5
rps16-trnQ-UUG_p4	GGATCGTGTCCTTCAAGTCG	GAAATTGAAATGGGGCGTGG	55.0	50.0	58.4	58.0	1233-1294 bp	0.964286	86.0
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.2	58.0	1211-1272 bp	0.964286	82.8