primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GGAACTTCCGTCTAATCAAACG	20	22	50.0	45.5	58.2	57.4	20.3	0.0	2.747690	4205	5094	944-974	1.000000	69.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GGAACTTCCGTCTAATCAAACG	20	22	50.0	45.5	58.2	57.4	20.3	0.0	2.747690	4205	5094	943-973	1.000000	69.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTAGCCGCACTTAAAAGCC	GGAACTTCCGTCTAATCAAACG	20	22	50.0	45.5	58.3	57.4	0.0	0.0	2.845107	4205	5094	958-988	1.000000	68.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCAGTCGTGGTCTTCCAAAC	GGAACTTCCGTCTAATCAAACG	20	22	50.0	45.5	57.8	57.4	0.0	0.0	2.813570	4205	5094	1029-1059	1.000000	67.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CAGTCGTGGTCTTCCAAACT	GGAACTTCCGTCTAATCAAACG	20	22	50.0	45.5	57.8	57.4	0.0	0.0	2.819763	4205	5094	1028-1058	1.000000	67.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6211	7326	1161-1222	1.000000	89.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	20	20	45.0	50.0	58.1	58.0	8.1	0.0	0.148076	6211	7326	1219-1280	0.964286	88.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6211	7326	1175-1236	1.000000	87.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GGATCGTGTCCTTCAAGTCG	GAAATTGAAATGGGGCGTGG	20	20	55.0	50.0	58.4	58.0	2.9	0.0	0.378604	6211	7326	1233-1294	0.964286	86.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	57.2	58.0	0.0	0.0	0.796474	6211	7326	1211-1272	0.964286	82.8
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	28389	29229	913-956	1.000000	88.5
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	28389	29229	911-954	1.000000	88.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.162414	28389	29229	914-957	1.000000	87.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTGTATCAGTTTGGCGGCAT	ACTTCTTCCCCACACTACGA	20	20	45.0	50.0	58.2	58.0	14.3	0.0	0.172487	28389	29229	967-1010	1.000000	87.7
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTGTATCAGTTTGGCGGCAT	TTCTTCCCCACACTACGAGT	20	20	45.0	50.0	58.2	58.0	14.3	0.0	0.172487	28389	29229	965-1008	1.000000	87.7
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	32087	33433	1461-1521	1.000000	84.0
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	32087	33433	1464-1524	1.000000	83.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	32087	33433	1496-1531	0.964286	81.5
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	32087	33433	1499-1534	0.964286	81.3
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.395920	32087	33433	1473-1508	0.964286	79.8
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.263697	36715	37018	447-479	1.000000	87.6
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.263697	36715	37018	448-480	1.000000	87.5
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.314679	36715	37018	445-477	1.000000	87.2
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.314679	36715	37018	446-478	1.000000	87.2
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	CCGTCGTATTTGCTTCTCCT	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.353928	36715	37018	456-488	1.000000	86.7
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.961472	45088	45918	993-1026	1.000000	76.8
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.963464	45088	45918	992-1025	1.000000	76.8
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.018457	45088	45918	1014-1047	1.000000	76.2
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	GCCTCCTTTTCTCCTGAAGT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.020450	45088	45918	1013-1046	1.000000	76.2
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	57.1	57.8	0.0	5.6	1.059773	45088	45918	964-997	1.000000	76.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	47324	48025	758-803	1.000000	84.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	47324	48025	790-835	1.000000	83.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	47324	48025	752-797	1.000000	83.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	47324	48025	790-835	1.000000	82.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	47324	48025	784-829	1.000000	82.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	0.7	0.0	0.279423	51360	52510	1272-1288	0.964286	83.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.7	0.0	0.765489	51360	52510	1260-1281	1.000000	81.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TTCTCCGGCCCTTTCCTAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.4	57.8	0.0	0.0	0.605515	51360	52510	1293-1309	0.964286	80.4
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	0.7	0.0	0.623195	51360	52510	1271-1287	0.964286	80.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.6	57.8	0.0	0.0	0.766291	51360	52510	1285-1301	0.964286	79.2
ycf4-cemA_p1	ycf4-cemA	1	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	61707	62580	916-1056	1.000000	86.4
ycf4-cemA_p2	ycf4-cemA	2	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129660	61707	62580	915-1055	1.000000	86.4
ycf4-cemA_p3	ycf4-cemA	3	TATTTCTTGCGCGTACCGAT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	8.4	0.0	0.289513	61707	62580	847-987	1.000000	85.2
ycf4-cemA_p4	ycf4-cemA	4	GAACAAAAAGCCGCGGAATT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.2	58.1	33.5	0.0	0.298310	61707	62580	871-1011	1.000000	85.2
ycf4-cemA_p5	ycf4-cemA	5	GCTATTCCCTTGACCCGTAC	GAGAAATCCACCAGGGCAAA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.312489	61707	62580	919-1059	1.000000	84.9
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.2	58.1	5.4	0.0	1.272430	64442	65375	1040-1136	1.000000	77.8
petA-psbJ_p2	petA-psbJ	2	TCGAGAAGGTTCAATTGGCC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.2	58.1	5.4	0.0	1.272430	64442	65375	1042-1138	1.000000	77.8
petA-psbJ_p3	petA-psbJ	3	GAGAAGGTTCAATTGGCCGA	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.2	57.8	5.4	0.0	1.364030	64442	65375	1041-1137	1.000000	77.0
petA-psbJ_p4	petA-psbJ	4	TCGAGAAGGTTCAATTGGCC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.2	57.8	5.4	0.0	1.364030	64442	65375	1043-1139	1.000000	77.0
petA-psbJ_p5	petA-psbJ	5	GATCCATTACGGGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	0.0	0.0	1.349763	64442	65375	1127-1223	1.000000	76.7
ndhF_p1	ndhF	1	AGGGTGGAAAGTGAGGAAGA	GCCCAATACCCCTTCCTTTT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.366102	110611	112866	2939-2975	0.500000	59.4
ndhF_p2	ndhF	2	CGGAAGAAAGGGTGGAAAGT	GCCCAATACCCCTTCCTTTT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.519643	110611	112866	2947-2983	0.500000	58.2
ndhF_p3	ndhF	3	AGGGTGGAAAGTGAGGAAGA	TCCCCAAGGAAAACGCTTTT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.335332	110611	112866	2965-2995	0.464286	58.1
ndhF_p4	ndhF	4	ACGGAAGAAAGGGTGGAAAG	TCCCCAAGGAAAACGCTTTT	20	20	50.0	45.0	57.7	58.2	0.0	0.0	0.488873	110611	112866	2974-2987	0.428571	55.4
ndhF_p5	ndhF	5	CGGAAGAAAGGGTGGAAAGT	TCCCCAAGGAAAACGCTTTT	20	20	50.0	45.0	57.7	58.2	0.0	0.0	0.488873	110611	112866	2973-2986	0.428571	55.4
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAAGGTATGATCCACGCA	TGATGCAGAAGTACGTTTTT	20	20	50.0	35.0	57.7	53.5	0.0	0.0	4.861698	112867	113372	569-594	1.000000	51.9
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAAGGTATGATCCACGCA	TTGATGCAGAAGTACGTTTT	20	20	50.0	35.0	57.7	53.5	0.0	0.0	4.861698	112867	113372	570-595	1.000000	51.9
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAAGGTATGATCCACGCA	TTTGATGCAGAAGTACGTTT	20	20	50.0	35.0	57.7	53.5	0.0	0.0	4.861698	112867	113372	571-596	1.000000	51.9
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAAGGTATGATCCACGCA	TTTTGATGCAGAAGTACGTT	20	20	50.0	35.0	57.7	53.5	0.0	0.0	4.861698	112867	113372	572-597	1.000000	51.9
ndhF-rpl32_p5	ndhF-rpl32	5	GGAAAGGTATGATCCACGCA	TTTTTGATGCAGAAGTACGT	20	20	50.0	35.0	57.7	53.5	0.0	0.0	4.861698	112867	113372	573-598	1.000000	51.9
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GTGCAACAAACAAATAAAATAAGT	TCCTAAGAGCAGCGTGTCTA	24	20	25.0	50.0	53.6	57.9	0.0	0.0	8.539213	113550	114447	951-998	0.964286	49.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GTGCAACAAACAAATAAAATAAGT	AGCAGCGTGTCTACCAATTT	24	20	25.0	45.0	53.6	57.8	0.0	0.0	8.599940	113550	114447	944-991	0.964286	49.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GTGCAACAAACAAATAAAATAAGT	CCTAAGAGCAGCGTGTCTAC	24	20	25.0	55.0	53.6	57.8	0.0	0.0	8.611231	113550	114447	950-997	0.964286	49.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GTGCAACAAACAAATAAAATAAGT	CTAAGAGCAGCGTGTCTACC	24	20	25.0	55.0	53.6	57.8	0.0	0.0	8.611231	113550	114447	949-996	0.964286	49.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GTGCAACAAACAAATAAAATAAGT	TTTAGGAGATGACATGCCGC	24	20	25.0	50.0	53.6	58.0	0.0	0.0	8.461377	113550	114447	1006-1053	0.964286	49.1
ccsA-ndhD_p1	ccsA-ndhD	1	TTCAGCAATTGTGGCGTCTA	ACCCCGATTTCGTTCTTTCA	20	20	45.0	45.0	57.8	57.4	7.4	0.0	0.731764	115595	115834	409-430	1.000000	84.4
ccsA-ndhD_p2	ccsA-ndhD	2	GCAATTGTGGCGTCTATTGG	ACCCCGATTTCGTTCTTTCA	20	20	50.0	45.0	57.8	57.4	7.4	0.0	0.747676	115595	115834	405-426	1.000000	84.3
ccsA-ndhD_p3	ccsA-ndhD	3	AATTCAGCAATTGTGGCGTC	ACCCCGATTTCGTTCTTTCA	20	20	45.0	45.0	57.6	57.4	7.4	0.0	0.906873	115595	115834	411-432	1.000000	82.9
ccsA-ndhD_p4	ccsA-ndhD	4	TCAGCAATTGTGGCGTCTAT	ACCCCGATTTCGTTCTTTCA	20	20	45.0	45.0	57.6	57.4	7.4	0.0	0.955953	115595	115834	408-429	1.000000	82.6
ccsA-ndhD_p5	ccsA-ndhD	5	ATTCAGCAATTGTGGCGTCT	ACCCCGATTTCGTTCTTTCA	20	20	45.0	45.0	58.5	57.4	7.4	0.0	1.016323	115595	115834	410-431	1.000000	82.1
ndhD-psaC_p1	ndhD-psaC	1	GAACCCGCAAATATTGGCAA	CCGACGGATTTCTTGAGTGT	20	20	45.0	50.0	57.3	57.9	15.1	8.6	0.744112	117338	117461	252-263	1.000000	82.8
ndhD-psaC_p2	ndhD-psaC	2	GAACCCGCAAATATTGGCAA	ATGGTCTGAAACAACTCGCA	20	20	45.0	45.0	57.3	57.7	15.1	0.0	0.918372	117338	117461	220-231	1.000000	82.4
ndhD-psaC_p3	ndhD-psaC	3	AGGAACCCGCAAATATTGGC	CCGACGGATTTCTTGAGTGT	20	20	50.0	50.0	58.9	57.9	4.6	8.6	0.969009	117338	117461	254-265	1.000000	81.0
ndhD-psaC_p4	ndhD-psaC	4	GAACCCGCAAATATTGGCAA	TCCGACGGATTTCTTGAGTG	20	20	45.0	50.0	57.3	57.6	15.1	13.9	1.027614	117338	117461	253-264	1.000000	80.5
ndhD-psaC_p5	ndhD-psaC	5	GAACCCGCAAATATTGGCAA	AAGAGATGTGAATCCGCCTG	20	20	45.0	50.0	57.3	57.7	15.1	0.0	0.986182	117338	117461	273-284	1.000000	80.2
ycf1_p1	ycf1	1	TTTTTATGTAGCGAAATGGTC	ACAGCAGAAGATCGTTCTCC	21	20	33.3	50.0	52.5	57.3	0.0	0.0	7.119113	128520	128639	178-178	1.000000	48.0
ycf1_p2	ycf1	2	TTTTTATGTAGCGAAATGGTC	AGGGTGGAAAGTGAGGAAGA	21	20	33.3	50.0	52.5	57.9	0.0	0.0	6.573690	128520	128639	217-217	0.678571	35.1
ycf1_p3	ycf1	3	TTTTTATGTAGCGAAATGGTC	ACGGAAGAAAGGGTGGAAAG	21	20	33.3	50.0	52.5	57.7	0.0	0.0	6.727231	128520	128639	226-226	0.678571	35.1
ycf1_p4	ycf1	4	TTTTTATGTAGCGAAATGGTC	CGGAAGAAAGGGTGGAAAGT	21	20	33.3	50.0	52.5	57.7	0.0	0.0	6.727231	128520	128639	225-225	0.678571	35.1
ycf1_p5	ycf1	5	TTTTTATGTAGCGAAATGGTC	AACGGAAGAAAGGGTGGAAA	21	20	33.3	45.0	52.5	57.3	0.0	0.0	7.195793	128520	128639	227-227	0.678571	35.1