Hotspot

rpl23

Genome · 45341-45982 · 642 bp

Back to Bryopsis

Pi 0.109

MarkerSeek score 79.80

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: Genome
length_bp: 642
variable_sites: 69
pi: 0.109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	CCCGAGTCCTCCTAGCATTA	ATGCATGTATGGCTGAGTGG	55.0	50.0	58.0	58.0	1460 bp	0.5	59.8
rpl23_p2	CCGTGTACCTGGTGTATGTG	ATGCATGTATGGCTGAGTGG	55.0	50.0	58.0	58.0	947 bp	0.5	59.8
rpl23_p3	CCCGAGTCCTCCTAGCATTA	AAAAGCGGCGGACTCATAAT	55.0	45.0	58.0	58.0	1438 bp	0.5	59.7
rpl23_p4	CCGTGTACCTGGTGTATGTG	AAAAGCGGCGGACTCATAAT	55.0	45.0	58.0	58.0	925 bp	0.5	59.7
rpl23_p5	CAAACGTTTATGCCCACCAC	ATGCATGTATGGCTGAGTGG	50.0	50.0	57.9	58.0	1088 bp	0.5	59.3