Hotspot

chlB

Genome · 49227-50744 · 1518 bp

Back to Bryopsis

Pi 0.102

MarkerSeek score 80.00

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: Genome
length_bp: 1518
variable_sites: 153
pi: 0.102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	CGGGATAGCTCAGTGGTAGA	AAGAAGTCCGCCTAATGCTG	55.0	50.0	58.0	58.0	2950-2951 bp	1.0	88.5
chlB_p2	GTGGGAGGATTCGAACCTTC	AAGAAGTCCGCCTAATGCTG	55.0	50.0	58.0	58.0	2831-2834 bp	1.0	88.4
chlB_p3	TCAAAAAGCCGGGATAGCTC	AAGAAGTCCGCCTAATGCTG	50.0	50.0	58.0	58.0	2959-2960 bp	1.0	88.4
chlB_p4	AAAAGAGCTGAGCTGCTTGT	AAGAAGTCCGCCTAATGCTG	45.0	50.0	58.0	58.0	2306-2307 bp	1.0	87.5
chlB_p5	ACACTTCAAAAAGCCGGGAT	AAGAAGTCCGCCTAATGCTG	45.0	50.0	58.0	58.0	2965 bp	0.5	59.7