Hotspot

cemA

Genome · 73294-74211 · 918 bp

Back to Bryopsis

Pi 0.0991

MarkerSeek score 80.30

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: Genome
length_bp: 918
variable_sites: 91
pi: 0.0991
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	ACGCACCTTTTGATCCACTT	AACAAGCGACCAGTGCTAAA	45.0	45.0	58.0	58.0	1594-1861 bp	1.0	86.3
cemA_p2	ACGCACCTTTTGATCCACTT	AAGCGACCAGTGCTAAACAA	45.0	45.0	58.0	58.0	1591-1858 bp	1.0	86.3
cemA_p3	ACGCACCTTTTGATCCACTT	AGCGACCAGTGCTAAACAAA	45.0	45.0	58.0	58.0	1590-1857 bp	1.0	86.3
cemA_p4	ACGCACCTTTTGATCCACTT	GCACGTGCTCGAAAAATTGA	45.0	45.0	58.0	58.0	2176 bp	0.5	59.6
cemA_p5	TGGCAAGCTTCGGTAAGTTT	GCACGTGCTCGAAAAATTGA	45.0	45.0	58.0	58.0	2105 bp	0.5	59.6