Hotspot

atpA

Genome · 26092-28599 · 2508 bp

Back to Bryopsis

Pi 0.134

MarkerSeek score 80.70

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: Genome
length_bp: 2508
variable_sites: 322
pi: 0.134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	GAGCTGATCTCCACGTTTGT	AGAAAATCGTTACGATGGACT	50.0	38.1	57.9	55.1	2865-2929 bp	1.0	59.5
atpA_p2	GAGCTGATCTCCACGTTTGT	TCGTTACGATGGACTATTTCA	50.0	38.1	57.9	54.5	2859-2923 bp	1.0	55.2
atpA_p3	TTAACTAACCGACGCTCTAC	AGGGAAAAAGAGGCCATTGA	45.0	45.0	54.7	57.0	2991 bp	0.5	25.5
atpA_p4	AATTAACTAACCGACGCTCT	AGGGAAAAAGAGGCCATTGA	40.0	45.0	54.5	57.0	2993 bp	0.5	24.2
atpA_p5	TGAAAATTAACTAACCGACGCTC	AGGGAAAAAGAGGCCATTGA	39.1	45.0	57.5	57.0	2997 bp	0.5	23.8