Hotspot

trnR-trnA

Genome · 127160-127241 · 82 bp

Back to Bryopsis

Pi 0.6

MarkerSeek score 52.80

Primer available yes

Label trnR-trnA

Feature Metadata

label_name: trnR-trnA
feature_type: igs
region: Genome
length_bp: 82
variable_sites: 3
pi: 0.6
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.06
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnA_p1	TAGACCGACGCCTTATCCAT	GCACAAAAGTTCTGCGATCC	50.0	50.0	58.0	58.0	841 bp	0.5	59.7
trnR-trnA_p2	CCACCTAGACTACGACGGAT	GCACAAAAGTTCTGCGATCC	55.0	50.0	58.0	58.0	965 bp	0.5	59.5
trnR-trnA_p3	CACCAGGCCTTCAGATCTTC	GCACAAAAGTTCTGCGATCC	55.0	50.0	58.0	58.0	1139 bp	0.5	59.5
trnR-trnA_p4	CAAGCCTTGTGAAAGAAGCG	GCACAAAAGTTCTGCGATCC	50.0	50.0	58.0	58.0	1228 bp	0.5	59.4
trnR-trnA_p5	GACCGACGCCTTATCCATTT	GCACAAAAGTTCTGCGATCC	50.0	50.0	58.1	58.0	839 bp	0.5	59.3