Hotspot

rrn16-trnS

Genome · 91918-98935 · 7018 bp

Back to Bryopsis

Pi 0.424

MarkerSeek score 67.50

Primer available yes

Label rrn16-trnS

Feature Metadata

label_name: rrn16-trnS
feature_type: igs
region: Genome
length_bp: 7018
variable_sites: 1581
pi: 0.424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.53
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnS_p1	CGATCGAATGATTGGGGGTT	TTAGTGAGCGCAATGGAGTC	50.0	50.0	58.0	58.0	1437 bp	0.5	59.7
rrn16-trnS_p2	CGATCGAATGATTGGGGGTT	GGGAATCCAGAAGTCAGCTG	50.0	55.0	58.0	58.0	1307 bp	0.5	59.5
rrn16-trnS_p3	CTTCTGATTTCGGAGTGCCA	TTAGTGAGCGCAATGGAGTC	50.0	50.0	57.9	58.0	1200 bp	0.5	59.1
rrn16-trnS_p4	TCGAATGATTGGGGGTTCAC	TTAGTGAGCGCAATGGAGTC	50.0	50.0	57.9	58.0	1434 bp	0.5	58.9
rrn16-trnS_p5	CACGCTTCAGCACACAATTT	TTAGTGAGCGCAATGGAGTC	45.0	50.0	57.9	58.0	1094 bp	0.5	58.9