Hotspot

chlL-ccsA

Genome · 66104-66580 · 477 bp

Back to Bryopsis

Pi 0.111

MarkerSeek score 81.00

Primer available yes

Label chlL-ccsA

Feature Metadata

label_name: chlL-ccsA
feature_type: igs
region: Genome
length_bp: 477
variable_sites: 50
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL-ccsA_p1	CTTCTAAAACGGGCATCGGA	AACGCAGGAATTAAGGGACC	50.0	50.0	58.0	57.9	1572 bp	0.5	58.9
chlL-ccsA_p2	CAACACAATCCACACCTCCA	AACGCAGGAATTAAGGGACC	50.0	50.0	58.0	57.9	1221 bp	0.5	58.8
chlL-ccsA_p3	GACCTCCTGCTTCAACACAA	AACGCAGGAATTAAGGGACC	50.0	50.0	58.0	57.9	1233 bp	0.5	58.7
chlL-ccsA_p4	ACAACGTAACCTCCACAACC	AACGCAGGAATTAAGGGACC	50.0	50.0	58.0	57.9	1267 bp	0.5	58.7
chlL-ccsA_p5	CAACGTAACCTCCACAACCA	AACGCAGGAATTAAGGGACC	50.0	50.0	58.0	57.9	1266 bp	0.5	58.7