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psbZ-psbD

Genome · 58009-59186 · 1178 bp

Back to Bryopsis

Pi 0.0997

MarkerSeek score 79.80

Primer available yes

Label psbZ-psbD

Feature Metadata

label_name: psbZ-psbD
feature_type: igs
region: Genome
length_bp: 1178
variable_sites: 117
pi: 0.0997
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-psbD_p1	AATGGGTTGGCTTCGTGATT	TCGAACCAACCTCTCTCTGT	45.0	50.0	58.1	58.0	1845-1848 bp	1.0	86.8
psbZ-psbD_p2	GTATGGGCGTGGATGTTCTT	TCGAACCAACCTCTCTCTGT	50.0	50.0	58.0	58.0	1763 bp	0.5	59.7
psbZ-psbD_p3	GTATGGGCGTGGATGTTCTT	TAGCGGCAACTCCAAAACTT	50.0	45.0	58.0	58.0	2248 bp	0.5	59.5
psbZ-psbD_p4	GTATGGGCGTGGATGTTCTT	ATCGCGTTATACGGTCGAAG	50.0	50.0	58.0	58.0	2147 bp	0.5	59.5
psbZ-psbD_p5	AATGGGTTGGCTTCGTGATT	TAGCGGCAACTCCAAAACTT	45.0	45.0	58.1	58.0	2333 bp	0.5	59.2