Hotspot

rpl36-infA

Genome · 39942-39995 · 54 bp

Back to Bryopsis

Pi 0.18
MarkerSeek score 79.80
Primer available yes
Label rpl36-infA

Feature Metadata

label_name
rpl36-infA
feature_type
igs
region
Genome
length_bp
54
variable_sites
9
pi
0.18
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-infA_p1 ACAGAGGCACGTATTTTCAT TATGATTTAACACGTGGGCG 40.0 45.0 55.1 56.2 121-125 bp 1.0 50.9
rpl36-infA_p2 ACAGAGGCACGTATTTTCAT ATATGATTTAACACGTGGGCG 40.0 42.9 55.1 56.8 122-126 bp 1.0 48.4
rpl36-infA_p3 TACAGAGGCACGTATTTTCA TATGATTTAACACGTGGGCG 40.0 45.0 54.4 56.2 122-126 bp 1.0 48.4
rpl36-infA_p4 ACAGAGGCACGTATTTTCAT CATATGATTTAACACGTGGGCG 40.0 45.5 55.1 58.1 123-127 bp 1.0 48.3
rpl36-infA_p5 ACAGAGGCACGTATTTTCAT CCATATGATTTAACACGTGGGC 40.0 45.5 55.1 57.8 124-128 bp 1.0 48.3