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rpl12-cysT

Genome · 33869-34550 · 682 bp

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Pi 0.141

MarkerSeek score 81.90

Primer available yes

Label rpl12-cysT

Feature Metadata

label_name: rpl12-cysT
feature_type: igs
region: Genome
length_bp: 682
variable_sites: 94
pi: 0.141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl12-cysT_p1	TGCCCGGTGAAGATCAAAAA	GACCAAGCCGCTTCTTCTAA	45.0	50.0	58.0	57.9	1370-1373 bp	1.0	88.1
rpl12-cysT_p2	TGCCCGGTGAAGATCAAAAA	CTAATGACCAAGCCGCTTCT	45.0	50.0	58.0	58.0	1378 bp	0.5	59.7
rpl12-cysT_p3	GGCGCGAAAGTCAAAATTCA	CTAATGACCAAGCCGCTTCT	45.0	50.0	58.0	58.0	1205 bp	0.5	59.4
rpl12-cysT_p4	CAGGCGCGAAAGTCAAAATT	CTAATGACCAAGCCGCTTCT	45.0	50.0	57.9	58.0	1207 bp	0.5	59.4
rpl12-cysT_p5	TGCCCGGTGAAGATCAAAAA	ATTCACCAATCGCACGAGAA	45.0	45.0	58.0	57.9	1480 bp	0.5	59.2