Hotspot

ycf1

SSC · 127684-133122 · 5439 bp

Back to Brunsvigia

Pi 0.00558
MarkerSeek score 45.00
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5439
variable_sites
56
pi
0.00558
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GCACAAATTCTGGGAAAAACCA GAGACTTGGACCAATACGCA 40.9 50.0 58.2 57.6 223 bp 1.0 61.5
ycf1_p2 GCACAAATTCTGGGAAAAACCA TGAGACTTGGACCAATACGC 40.9 50.0 58.2 57.6 224 bp 1.0 61.5
ycf1_p3 GCACAAATTCTGGGAAAAACC GAGACTTGGACCAATACGCA 42.9 50.0 56.5 57.6 223 bp 1.0 59.0
ycf1_p4 GCACAAATTCTGGGAAAAACC TGAGACTTGGACCAATACGC 42.9 50.0 56.5 57.6 224 bp 1.0 59.0
ycf1_p5 GGCACAAATTCTGGGAAAAAC GAGACTTGGACCAATACGCA 42.9 50.0 56.5 57.6 224 bp 1.0 59.0