Hotspot

ndhF

SSC · 113614-115854 · 2241 bp

Back to Brunsvigia

Pi 0.158

MarkerSeek score 81.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 698
pi: 0.158
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTTTTGAACGGACCCCATCG	TCCGATCAATCAGTTCCACA	50.0	45.0	58.8	56.6	2890-2893 bp	0.5	42.8
ndhF_p2	GATGGAATCGCCCATTGAGA	ATTGTTCCTTTCGAAAGCGA	50.0	40.0	57.7	56.0	2841-2844 bp	0.5	42.1
ndhF_p3	CCGCCCAGTTTATCGACTTT	ATTGTTCCTTTCGAAAGCGA	50.0	40.0	58.3	56.0	2716-2719 bp	0.5	42.1
ndhF_p4	GGATCCACCGAACAAGACAA	GCGATAAGAATTGGTGAATCGG	50.0	45.5	57.8	57.9	2985-2988 bp	0.5	41.8
ndhF_p5	ACAGGAACAAGAGGGATCCA	GCGATAAGAATTGGTGAATCGG	50.0	45.5	58.0	57.9	2998 bp	0.25	32.6