Hotspot

ndhI-ndhA

SSC · 123409-123496 · 88 bp

Back to Brunsvigia

Pi 0.0341

MarkerSeek score 62.70

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 88
variable_sites: 6
pi: 0.0341
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TTGGGCAATACTCGACACAG	ACCAGAAGCAGAGGAGGAAT	50.0	50.0	57.9	58.0	828 bp	1.0	80.3
ndhI-ndhA_p2	TTGGGCAATACTCGACACAG	TTTTGGGGGTGGAATCCTTG	50.0	50.0	57.9	58.0	920 bp	0.5	59.9
ndhI-ndhA_p3	GGGCAATACTCGACACAGTT	TTTTGGGGGTGGAATCCTTG	50.0	50.0	57.9	58.0	918 bp	0.5	59.9
ndhI-ndhA_p4	TTGGGCAATACTCGACACAG	GTTTTGGGGGTGGAATCCTT	50.0	50.0	57.9	58.0	921 bp	0.5	59.9
ndhI-ndhA_p5	GGGCAATACTCGACACAGTT	GTTTTGGGGGTGGAATCCTT	50.0	50.0	57.9	58.0	919 bp	0.5	59.9