Hotspot

ndhF-rpl32

SSC · 115855-116804 · 950 bp

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Pi 0.108

MarkerSeek score 82.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 950
variable_sites: 202
pi: 0.108
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAAGAGGGAATTGTGCGGAT	AATGCTTAGAACCGGCAGAC	50.0	50.0	58.0	58.3	2007-2009 bp	0.75	68.6
ndhF-rpl32_p2	AAGCGGATCGATCAAGTGTC	AATGCTTAGAACCGGCAGAC	50.0	50.0	58.1	58.3	1544-1546 bp	0.75	68.4
ndhF-rpl32_p3	TTTTTGACAAGCACTTGCCG	AATGCTTAGAACCGGCAGAC	45.0	50.0	58.1	58.3	1799-1801 bp	0.75	68.3
ndhF-rpl32_p4	ATTTAGCAACTGCACCGACA	AATGCTTAGAACCGGCAGAC	45.0	50.0	58.1	58.3	1989-1991 bp	0.75	68.0
ndhF-rpl32_p5	TAGCAACTGCACCGACAAAT	AATGCTTAGAACCGGCAGAC	45.0	50.0	58.1	58.3	1986-1988 bp	0.75	68.0