Hotspot

atpB-rbcL

LSC · 56425-57418 · 994 bp

Back to Brunsvigia

Pi 0.0195

MarkerSeek score 62.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 994
variable_sites: 32
pi: 0.0195
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCCGACTGGAACACTTAGAG	TTGCGGAGTTACTCGGAATG	55.0	50.0	58.0	58.0	1331-1487 bp	1.0	85.9
atpB-rbcL_p2	GCCGACTGGAACACTTAGAG	GTTGCGGAGTTACTCGGAAT	55.0	50.0	58.0	58.0	1332-1488 bp	1.0	85.9
atpB-rbcL_p3	GCCGACTGGAACACTTAGAG	CGGTCTCTCCAACGCATAAA	55.0	50.0	58.0	58.0	1846-2002 bp	1.0	85.4
atpB-rbcL_p4	GCCGACTGGAACACTTAGAG	ATCCTCCAGACGTAGAGCTC	55.0	55.0	58.0	58.0	1607-1763 bp	1.0	85.4
atpB-rbcL_p5	GCCGACTGGAACACTTAGAG	GATCCTCCAGACGTAGAGCT	55.0	55.0	58.0	58.0	1608-1764 bp	1.0	85.4