Hotspot

ycf3-trnS-GGA

LSC · 45660-46242 · 583 bp

Back to Brunsvigia

Pi 0.006

MarkerSeek score 62.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 7
pi: 0.006
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATCCGGGATCTAGGC	ACGGAAAGAGAGGGATTCGA	52.4	50.0	57.7	58.2	695-696 bp	1.0	69.5
ycf3-trnS-GGA_p2	CCATTTATCCGGGATCTAGGC	TTCCAATGCTACGCCTTGAA	52.4	45.0	57.7	57.8	644-645 bp	1.0	69.2
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	768-769 bp	1.0	66.8
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	769-770 bp	1.0	66.8
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	717-718 bp	1.0	66.6