Hotspot

psaA-ycf3

LSC · 43033-43682 · 650 bp

Back to Brunsvigia

Pi 0.00462

MarkerSeek score 64.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 6
pi: 0.00462
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	CGACCAAGCTGCTGAGTATT	50.0	50.0	57.9	58.0	814-829 bp	1.0	81.2
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	ACCAAGCTGCTGAGTATTGG	50.0	50.0	57.9	57.9	812-827 bp	1.0	80.3
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	741-756 bp	1.0	80.2
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	745-760 bp	1.0	79.9
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	740-755 bp	1.0	79.2