primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	atpF	1	TCTTTTCGGAAACAAAGGGA	AGTCCTATCTATAAGAGGAGAGCA	20	24	40.0	41.7	55.1	57.3	2.0	0.0	7.618808	12184	13622	1515-1540	0.750000	31.9
atpF_p2	atpF	2	TCTTTTCGGAAACAAAGGGAA	AGTCCTATCTATAAGAGGAGAGCA	21	24	38.1	41.7	55.9	57.3	2.0	0.0	7.799783	12184	13622	1515-1540	0.750000	31.9
atpF_p3	atpF	3	TTCTTTTCGGAAACAAAGGGA	AGTCCTATCTATAAGAGGAGAGCA	21	24	38.1	41.7	55.9	57.3	2.0	0.0	7.799783	12184	13622	1516-1541	0.750000	31.9
atpF_p4	atpF	4	TCTTTTCGGAAACAAAGGGA	GTCCTATCTATAAGAGGAGAGCA	20	23	40.0	43.5	55.1	55.9	2.0	0.0	7.997922	12184	13622	1514-1539	0.750000	31.9
atpF_p5	atpF	5	TCTTTTCGGAAACAAAGGGAAA	AGTCCTATCTATAAGAGGAGAGCA	22	24	36.4	41.7	56.7	57.3	2.0	0.0	8.054995	12184	13622	1515-1540	0.750000	31.9
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.156700	13623	14119	620-621	1.000000	80.0
atpF-atpH_p2	atpF-atpH	2	CAGTGGCCTAAGGAAACGAA	ATTTATGGACTGGTCGTGGC	20	20	50.0	50.0	57.8	58.0	3.3	0.0	0.217299	13623	14119	592-593	1.000000	79.6
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	20	20	50.0	45.0	57.9	58.3	0.0	18.7	0.390245	13623	14119	607-608	1.000000	78.2
atpF-atpH_p4	atpF-atpH	4	CAGTGGCCTAAGGAAACGAA	TCGTGGCATTAGCGCTTTTA	20	20	50.0	45.0	57.8	58.3	3.3	18.7	0.450844	13623	14119	579-580	1.000000	77.8
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	CGTGGCATTAGCGCTTTTAT	20	20	50.0	45.0	57.9	57.2	0.0	18.7	0.874150	13623	14119	606-607	1.000000	74.3
psaA-ycf3_p1	psaA-ycf3	1	CCCATTCCTCGAAAGACGTT	CGACCAAGCTGCTGAGTATT	20	20	50.0	50.0	57.9	58.0	0.0	1.4	0.110498	43033	43682	814-829	1.000000	81.2
psaA-ycf3_p2	psaA-ycf3	2	CCCATTCCTCGAAAGACGTT	ACCAAGCTGCTGAGTATTGG	20	20	50.0	50.0	57.9	57.9	0.0	12.9	0.214563	43033	43682	812-827	1.000000	80.3
psaA-ycf3_p3	psaA-ycf3	3	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.258580	43033	43682	741-756	1.000000	80.2
psaA-ycf3_p4	psaA-ycf3	4	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.286351	43033	43682	745-760	1.000000	79.9
psaA-ycf3_p5	psaA-ycf3	5	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.384749	43033	43682	740-755	1.000000	79.2
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CCATTTATCCGGGATCTAGGC	ACGGAAAGAGAGGGATTCGA	21	20	52.4	50.0	57.7	58.2	14.0	0.0	1.419371	45660	46242	695-696	1.000000	69.5
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CCATTTATCCGGGATCTAGGC	TTCCAATGCTACGCCTTGAA	21	20	52.4	45.0	57.7	57.8	14.0	5.6	1.460686	45660	46242	644-645	1.000000	69.2
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	45660	46242	768-769	1.000000	66.8
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	45660	46242	769-770	1.000000	66.8
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	20	20	45.0	45.0	56.4	57.8	0.0	5.6	1.776834	45660	46242	717-718	1.000000	66.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47646	48518	871-931	1.000000	84.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AAAGCCAGCTATCGGAATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.133544	47646	48518	916-976	1.000000	84.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAAGCCAGCTATCGGAATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.198352	47646	48518	924-984	1.000000	84.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47646	48518	879-939	1.000000	84.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47646	48518	903-963	1.000000	83.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	51751	53465	1842-1873	1.000000	78.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAACTCCGGAAAT	AGCCCAATGCAACAAAATGC	20	20	50.0	45.0	57.9	58.5	5.2	0.0	0.613383	51751	53465	1924-1955	1.000000	76.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCCTTCACTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.662079	51751	53465	1855-1886	1.000000	75.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.9	57.5	5.2	0.0	0.680379	51751	53465	1841-1872	1.000000	75.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTGGTCCTTCACTAATCGGG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	57.4	57.8	0.0	0.0	0.801329	51751	53465	1859-1890	1.000000	74.7
atpB-rbcL_p1	atpB-rbcL	1	GCCGACTGGAACACTTAGAG	TTGCGGAGTTACTCGGAATG	20	20	55.0	50.0	58.0	58.0	0.0	21.4	0.006913	56425	57418	1331-1487	1.000000	85.9
atpB-rbcL_p2	atpB-rbcL	2	GCCGACTGGAACACTTAGAG	GTTGCGGAGTTACTCGGAAT	20	20	55.0	50.0	58.0	58.0	0.0	21.4	0.008301	56425	57418	1332-1488	1.000000	85.9
atpB-rbcL_p3	atpB-rbcL	3	GCCGACTGGAACACTTAGAG	CGGTCTCTCCAACGCATAAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.006913	56425	57418	1846-2002	1.000000	85.4
atpB-rbcL_p4	atpB-rbcL	4	GCCGACTGGAACACTTAGAG	ATCCTCCAGACGTAGAGCTC	20	20	55.0	55.0	58.0	58.0	0.0	5.3	0.026671	56425	57418	1607-1763	1.000000	85.4
atpB-rbcL_p5	atpB-rbcL	5	GCCGACTGGAACACTTAGAG	GATCCTCCAGACGTAGAGCT	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026671	56425	57418	1608-1764	1.000000	85.4
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	CCCAACCAGAAACTAGAGCC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.116294	74399	74831	608-609	1.000000	80.5
clpP-psbB_p2	clpP-psbB	2	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.116294	74399	74831	610-611	1.000000	80.5
clpP-psbB_p3	clpP-psbB	3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.238244	74399	74831	528-529	1.000000	79.8
clpP-psbB_p4	clpP-psbB	4	CGCACTATACGTCAACCCAA	CCAACCAGAAACTAGAGCCG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.274098	74399	74831	607-608	1.000000	79.3
clpP-psbB_p5	clpP-psbB	5	ACCCAAACTGCATCTTCCTC	CCCAACCAGAAACTAGAGCC	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.321919	74399	74831	594-595	1.000000	78.9
trnH-GUG-rpl2_p1	trnH-GUG-rpl2	1	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.157457	86856	86902	183-183	1.000000	80.5
trnH-GUG-rpl2_p2	trnH-GUG-rpl2	2	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	86856	86902	182-182	1.000000	80.5
trnH-GUG-rpl2_p3	trnH-GUG-rpl2	3	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	57.9	58.0	0.0	22.4	0.158965	86856	86902	184-184	1.000000	80.5
trnH-GUG-rpl2_p4	trnH-GUG-rpl2	4	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.1	58.0	0.2	22.4	0.153095	86856	86902	197-197	1.000000	80.4
trnH-GUG-rpl2_p5	trnH-GUG-rpl2	5	CGGATGTAGCCAAGTGGATC	TATCCTGCGCTTGGAAGAAG	20	20	55.0	50.0	58.1	57.7	0.2	22.4	0.444845	86856	86902	195-195	1.000000	78.1
ndhF_p1	ndhF	1	TTTTTGAACGGACCCCATCG	TCCGATCAATCAGTTCCACA	20	20	50.0	45.0	58.8	56.6	0.0	0.0	2.200752	113614	115854	2890-2893	0.500000	42.8
ndhF_p2	ndhF	2	GATGGAATCGCCCATTGAGA	ATTGTTCCTTTCGAAAGCGA	20	20	50.0	40.0	57.7	56.0	0.0	28.7	2.276834	113614	115854	2841-2844	0.500000	42.1
ndhF_p3	ndhF	3	CCGCCCAGTTTATCGACTTT	ATTGTTCCTTTCGAAAGCGA	20	20	50.0	40.0	58.3	56.0	0.0	28.7	2.281828	113614	115854	2716-2719	0.500000	42.1
ndhF_p4	ndhF	4	GGATCCACCGAACAAGACAA	GCGATAAGAATTGGTGAATCGG	20	22	50.0	45.5	57.8	57.9	7.0	0.0	2.312768	113614	115854	2985-2988	0.500000	41.8
ndhF_p5	ndhF	5	ACAGGAACAAGAGGGATCCA	GCGATAAGAATTGGTGAATCGG	20	22	50.0	45.5	58.0	57.9	15.2	0.0	2.178649	113614	115854	2998-2998	0.250000	32.6
ndhF-rpl32_p1	ndhF-rpl32	1	GAAGAGGGAATTGTGCGGAT	AATGCTTAGAACCGGCAGAC	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.305056	115855	116804	2007-2009	0.750000	68.6
ndhF-rpl32_p2	ndhF-rpl32	2	AAGCGGATCGATCAAGTGTC	AATGCTTAGAACCGGCAGAC	20	20	50.0	50.0	58.1	58.3	25.9	0.0	0.333038	115855	116804	1544-1546	0.750000	68.4
ndhF-rpl32_p3	ndhF-rpl32	3	TTTTTGACAAGCACTTGCCG	AATGCTTAGAACCGGCAGAC	20	20	45.0	50.0	58.1	58.3	10.3	0.0	0.341015	115855	116804	1799-1801	0.750000	68.3
ndhF-rpl32_p4	ndhF-rpl32	4	ATTTAGCAACTGCACCGACA	AATGCTTAGAACCGGCAGAC	20	20	45.0	50.0	58.1	58.3	4.2	0.0	0.370057	115855	116804	1989-1991	0.750000	68.0
ndhF-rpl32_p5	ndhF-rpl32	5	TAGCAACTGCACCGACAAAT	AATGCTTAGAACCGGCAGAC	20	20	45.0	50.0	58.1	58.3	4.2	0.0	0.370057	115855	116804	1986-1988	0.750000	68.0
ndhD_p1	ndhD	1	AAGACTTTTCTTTCTGTCTCA	CAAAAGCCCGTACTCGAGAA	21	20	33.3	50.0	52.6	57.9	0.0	21.7	6.430430	119070	120570	1610-1618	1.000000	41.8
ndhD_p2	ndhD	2	AAGACTTTTCTTTCTGTCTCA	CCTTTTTACCGGCAAAAGCC	21	20	33.3	50.0	52.6	58.2	0.0	24.7	6.565128	119070	120570	1622-1630	1.000000	41.8
ndhD_p3	ndhD	3	AAGACTTTTCTTTCTGTCTCA	CTTTTTACCGGCAAAAGCCC	21	20	33.3	50.0	52.6	58.2	0.0	20.8	6.565128	119070	120570	1621-1629	1.000000	41.8
ndhD_p4	ndhD	4	AGACTTTTCTTTCTGTCTCAT	CAAAAGCCCGTACTCGAGAA	21	20	33.3	50.0	52.4	57.9	0.0	21.7	6.667593	119070	120570	1609-1617	1.000000	41.8
ndhD_p5	ndhD	5	AGACTTTTCTTTCTGTCTCATT	CAAAAGCCCGTACTCGAGAA	22	20	31.8	50.0	53.3	57.9	0.0	21.7	6.758898	119070	120570	1609-1617	1.000000	41.8
ndhI-ndhA_p1	ndhI-ndhA	1	TTGGGCAATACTCGACACAG	ACCAGAAGCAGAGGAGGAAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.129321	123409	123496	828-828	1.000000	80.3
ndhI-ndhA_p2	ndhI-ndhA	2	TTGGGCAATACTCGACACAG	TTTTGGGGGTGGAATCCTTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.116024	123409	123496	920-920	0.500000	59.9
ndhI-ndhA_p3	ndhI-ndhA	3	GGGCAATACTCGACACAGTT	TTTTGGGGGTGGAATCCTTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.117480	123409	123496	918-918	0.500000	59.9
ndhI-ndhA_p4	ndhI-ndhA	4	TTGGGCAATACTCGACACAG	GTTTTGGGGGTGGAATCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.117482	123409	123496	921-921	0.500000	59.9
ndhI-ndhA_p5	ndhI-ndhA	5	GGGCAATACTCGACACAGTT	GTTTTGGGGGTGGAATCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.118938	123409	123496	919-919	0.500000	59.9
ycf1_p1	ycf1	1	GCACAAATTCTGGGAAAAACCA	GAGACTTGGACCAATACGCA	22	20	40.9	50.0	58.2	57.6	0.0	7.5	2.577217	128779	128898	223-223	1.000000	61.5
ycf1_p2	ycf1	2	GCACAAATTCTGGGAAAAACCA	TGAGACTTGGACCAATACGC	22	20	40.9	50.0	58.2	57.6	0.0	7.5	2.577217	128779	128898	224-224	1.000000	61.5
ycf1_p3	ycf1	3	GCACAAATTCTGGGAAAAACC	GAGACTTGGACCAATACGCA	21	20	42.9	50.0	56.5	57.6	0.0	7.5	2.894282	128779	128898	223-223	1.000000	59.0
ycf1_p4	ycf1	4	GCACAAATTCTGGGAAAAACC	TGAGACTTGGACCAATACGC	21	20	42.9	50.0	56.5	57.6	0.0	7.5	2.894282	128779	128898	224-224	1.000000	59.0
ycf1_p5	ycf1	5	GGCACAAATTCTGGGAAAAAC	GAGACTTGGACCAATACGCA	21	20	42.9	50.0	56.5	57.6	0.0	7.5	2.894282	128779	128898	224-224	1.000000	59.0
rpl2-trnH-GUG_p1	rpl2-trnH-GUG	1	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.157457	158339	158385	183-183	1.000000	80.5
rpl2-trnH-GUG_p2	rpl2-trnH-GUG	2	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	158339	158385	182-182	1.000000	80.5
rpl2-trnH-GUG_p3	rpl2-trnH-GUG	3	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.0	57.9	22.4	0.0	0.158965	158339	158385	184-184	1.000000	80.5
rpl2-trnH-GUG_p4	rpl2-trnH-GUG	4	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.0	58.1	22.4	0.2	0.153095	158339	158385	197-197	1.000000	80.4
rpl2-trnH-GUG_p5	rpl2-trnH-GUG	5	TATCCTGCGCTTGGAAGAAG	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	57.7	58.1	22.4	0.2	0.444845	158339	158385	195-195	1.000000	78.1