Hotspot

ycf1

SSC · 127146-132866 · 5721 bp

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Pi 0.00806

MarkerSeek score 56.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5721
variable_sites: 46
pi: 0.00806
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAATCAAGCTCTGCTTCCGT	AGGATCTTTTTATATCATCGCATGA	45.0	32.0	57.8	56.5	214 bp	1.0	41.5
ycf1_p2	AAGCTCTGCTTCCGTGTTAC	AGGATCTTTTTATATCATCGCATGA	50.0	32.0	58.2	56.5	209 bp	1.0	41.5
ycf1_p3	CAAGCTCTGCTTCCGTGTTA	AGGATCTTTTTATATCATCGCATGA	50.0	32.0	58.2	56.5	210 bp	1.0	41.5
ycf1_p4	AAATCAAGCTCTGCTTCCGT	GAGGATCTTTTTATATCATCGCATGA	45.0	34.6	57.8	57.5	215 bp	1.0	41.5
ycf1_p5	AAGCTCTGCTTCCGTGTTAC	GAGGATCTTTTTATATCATCGCATGA	50.0	34.6	58.2	57.5	210 bp	1.0	41.5