Hotspot

ndhD-psaC

SSC · 120318-120457 · 140 bp

Back to Brunellia

Pi 0.0504

MarkerSeek score 78.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 140
variable_sites: 7
pi: 0.0504
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AAAATTAAGGAACCCGCGGA	AAGATGTGAATCCGCCTGTC	45.0	50.0	57.8	58.0	296-298 bp	1.0	80.3
ndhD-psaC_p2	AAAATTAAGGAACCCGCGGA	TTATGGCATGAAACAACGCG	45.0	45.0	57.8	57.7	247-249 bp	1.0	78.9
ndhD-psaC_p3	AAATTAAGGAACCCGCGGAT	AAGATGTGAATCCGCCTGTC	45.0	50.0	57.6	58.0	295-297 bp	1.0	78.5
ndhD-psaC_p4	AAAATTAAGGAACCCGCGGA	CAGAGGACTGTGTTGGTTGT	45.0	50.0	57.8	57.7	318-320 bp	1.0	77.7
ndhD-psaC_p5	GAAAATTAAGGAACCCGCGG	AAGATGTGAATCCGCCTGTC	50.0	50.0	57.4	58.0	297-299 bp	1.0	77.5