Hotspot

rps8-rpl14

LSC · 83882-84101 · 220 bp

Back to Brunellia

Pi 0.0376

MarkerSeek score 74.10

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 8
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ATTCGAACCGCTCCTTTTCT	GGAAATCCAAAAGGAACGCG	45.0	50.0	57.5	58.0	389-396 bp	1.0	79.1
rps8-rpl14_p2	ATTCGAACCGCTCCTTTTCT	CGAATTTTTGGTGCGATCCC	45.0	50.0	57.5	58.1	371-378 bp	1.0	78.7
rps8-rpl14_p3	ATTCGAACCGCTCCTTTTCT	GAATTTTTGGTGCGATCCCC	45.0	50.0	57.5	57.7	370-377 bp	1.0	77.1
rps8-rpl14_p4	ATTCGAACCGCTCCTTTTCT	AGGAACGCGAATTTTTGGTG	45.0	45.0	57.5	57.6	378-385 bp	1.0	76.0
rps8-rpl14_p5	TTCGAACCGCTCCTTTTCTA	GGAAATCCAAAAGGAACGCG	45.0	50.0	56.9	58.0	388-395 bp	1.0	74.0