Hotspot

infA-rps8

LSC · 83350-83476 · 127 bp

Back to Brunellia

Pi 0.0394

MarkerSeek score 65.40

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 5
pi: 0.0394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CCGTTGGGAAGTGATTCAGT	CGGGAGGGGGATTGTAATTC	50.0	55.0	57.8	57.7	286 bp	1.0	77.7
infA-rps8_p2	CCGTTGGGAAGTGATTCAGT	TCGGCGGAGAAATTTTGTGT	50.0	45.0	57.8	58.4	215 bp	1.0	77.2
infA-rps8_p3	CCGTTGGGAAGTGATTCAGT	CGGCGGAGAAATTTTGTGTT	50.0	45.0	57.8	57.6	214 bp	1.0	77.1
infA-rps8_p4	CCGTTGGGAAGTGATTCAGT	CGGGGTATAATGACAGACCG	50.0	55.0	57.8	57.6	255 bp	1.0	76.6
infA-rps8_p5	CCGTTGGGAAGTGATTCAGT	TCGACTAGAAAGAATCGGCG	50.0	50.0	57.8	57.5	229 bp	1.0	76.5