Hotspot

rpl20-rps12

LSC · 72220-73038 · 819 bp

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Pi 0.00641

MarkerSeek score 67.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 5
pi: 0.00641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTTGTTCTACGTCTCCGAGC	CGCCGAGGAACATGTACTAG	50.0	55.0	57.7	57.9	855-893 bp	1.0	80.3
rpl20-rps12_p2	GTAAGTCGTGAATGAGCCCC	CGCCGAGGAACATGTACTAG	55.0	55.0	58.3	57.9	903-941 bp	1.0	80.1
rpl20-rps12_p3	TGTTCTACGTCTCCGAGCTA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.6	57.9	853-891 bp	1.0	79.9
rpl20-rps12_p4	TTTGTTCTACGTCTCCGAGC	AGCCAATCAGAACTGTCACG	50.0	50.0	57.7	58.2	905-943 bp	1.0	79.5
rpl20-rps12_p5	GTAAGTCGTGAATGAGCCCC	AGCCAATCAGAACTGTCACG	55.0	50.0	58.3	58.2	953-991 bp	1.0	79.3