Hotspot

petA-psbJ

LSC · 66005-67073 · 1069 bp

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Pi 0.0159

MarkerSeek score 67.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 17
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1214-1234 bp	1.0	77.9
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1197-1217 bp	1.0	77.0
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1211-1231 bp	1.0	76.8
petA-psbJ_p4	GCCTTTTGTTCTTCTTGGCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1196-1216 bp	1.0	76.4
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	CCGGTATTCTTGTGATCGGT	50.0	50.0	58.0	57.4	1262-1282 bp	1.0	76.0