Hotspot

rbcL-accD

LSC · 59628-60359 · 732 bp

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Pi 0.0137

MarkerSeek score 67.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 10
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	AGCCCACACCTGTATTTGTA	55.0	45.0	58.0	56.4	890-897 bp	1.0	68.4
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TAGCCCACACCTGTATTTGT	55.0	45.0	58.0	56.4	891-898 bp	1.0	68.4
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	TTTACTTAGCCCACACCTGT	55.0	45.0	58.0	56.4	897-904 bp	1.0	67.8
rbcL-accD_p4	CGCTGCTTGTGAAGTATGGA	AGCCCACACCTGTATTTGTA	50.0	45.0	58.3	56.4	861-868 bp	1.0	66.5
rbcL-accD_p5	CGCTGCTTGTGAAGTATGGA	TAGCCCACACCTGTATTTGT	50.0	45.0	58.3	56.4	862-869 bp	1.0	66.5