Hotspot

trnF-GAA-ndhJ

LSC · 51499-52243 · 745 bp

Back to Brunellia

Pi 0.00825

MarkerSeek score 56.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 6
pi: 0.00825
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	833-851 bp	1.0	81.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GATAATCATCCGCGCCTGAA	50.0	50.0	58.1	58.2	872-890 bp	1.0	80.1
trnF-GAA-ndhJ_p3	AATGGTCGGGATAGCTCAGT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.2	58.1	876-894 bp	1.0	79.8
trnF-GAA-ndhJ_p4	ATGGTCGGGATAGCTCAGTT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.2	58.1	875-893 bp	1.0	79.8
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATAATCATCCGCGCCTGAAA	50.0	45.0	58.1	57.7	871-889 bp	1.0	79.7