Hotspot

rpoB-trnC-GCA

LSC · 28188-29408 · 1221 bp

Back to Brunellia

Pi 0.00604

MarkerSeek score 56.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1221
variable_sites: 7
pi: 0.00604
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCATTTCCATCCCCGAGC	CGGACTTGAACTGGGGAAAA	57.9	50.0	58.0	58.0	1244-1303 bp	1.0	76.0
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCC	CGGACTTGAACTGGGGAAAA	50.0	50.0	56.9	58.0	1249-1308 bp	1.0	75.5
rpoB-trnC-GCA_p3	TCATTTCCATCCCCGAGCAT	CGGACTTGAACTGGGGAAAA	50.0	50.0	59.2	58.0	1243-1302 bp	1.0	74.9
rpoB-trnC-GCA_p4	ACATTCCCTCATTTCCATCCC	CGGACTTGAACTGGGGAAAA	47.6	50.0	57.7	58.0	1251-1310 bp	1.0	73.6
rpoB-trnC-GCA_p5	CTCATTTCCATCCCCGAGC	AAAAGGATTTGCAGTCCCCC	57.9	50.0	58.0	58.4	1227-1286 bp	1.0	73.4