Hotspot

trnG-UCC-trnR-UCU

LSC · 10448-10799 · 352 bp

Back to Brunellia

Pi 0.0176

MarkerSeek score 61.50

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 6
pi: 0.0176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	430-435 bp	1.0	66.4
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	436-441 bp	1.0	65.7
trnG-UCC-trnR-UCU_p3	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.7	56.9	431-436 bp	1.0	64.2
trnG-UCC-trnR-UCU_p4	CCCTAGCCTTCCAAGCTAAC	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	57.7	57.9	437-442 bp	1.0	63.5
trnG-UCC-trnR-UCU_p5	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCAT	55.0	45.5	58.1	57.4	430-435 bp	1.0	62.5