Hotspot

rps15-trnN-GUU

SSC · 126384-133100 · 6717 bp

Back to Bruinsmia

Pi 0.0407

MarkerSeek score 77.60

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: SSC
length_bp: 6717
variable_sites: 268
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	ATGCTCTGCTGGCATTACTC	AAGATCCTTCTTCTGCCCCT	50.0	50.0	58.0	58.0	1136-1163 bp	1.0	88.0
rps15-trnN-GUU_p2	ATGCTCTGCTGGCATTACTC	AGATCCTTCTTCTGCCCCTT	50.0	50.0	58.0	58.0	1135-1162 bp	1.0	88.0
rps15-trnN-GUU_p3	ATGCTCTGCTGGCATTACTC	ACGAAGGGGACTAAACAGGA	50.0	50.0	58.0	58.0	1176-1203 bp	1.0	87.8
rps15-trnN-GUU_p4	CTTTGGGCTCGGATAGAACC	ACGAAGGGGACTAAACAGGA	55.0	50.0	58.0	58.0	1373-1424 bp	1.0	86.8
rps15-trnN-GUU_p5	GGGCTCGGATAGAACCTTTG	ACGAAGGGGACTAAACAGGA	55.0	50.0	58.0	58.0	1369-1420 bp	1.0	86.8