Hotspot

ndhI-ndhA

SSC · 122743-122842 · 100 bp

Back to Bruinsmia

Pi 0.07

MarkerSeek score 83.80

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 100
variable_sites: 7
pi: 0.07
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGCAGCTCGTACTGTTTGTT	GATGGACTTTACCGAGGCTG	45.0	55.0	58.1	58.1	276 bp	1.0	81.1
ndhI-ndhA_p2	TTGCAGCTCGTACTGTTTGT	GATGGACTTTACCGAGGCTG	45.0	55.0	58.1	58.1	277 bp	1.0	81.1
ndhI-ndhA_p3	TGCAGCTCGTACTGTTTGTT	TTACCGAGGCTGAGAATGGA	45.0	50.0	58.1	58.1	268 bp	1.0	80.6
ndhI-ndhA_p4	TGCAGCTCGTACTGTTTGTT	AGATGGACTTTACCGAGGCT	45.0	50.0	58.1	58.1	277 bp	1.0	80.5
ndhI-ndhA_p5	TTGCAGCTCGTACTGTTTGT	AGATGGACTTTACCGAGGCT	45.0	50.0	58.1	58.1	278 bp	1.0	80.5