Hotspot

trnN-GUU-ndhF

IRb · 111275-113081 · 1807 bp

Back to Bruinsmia

Pi 0.0602

MarkerSeek score 89.60

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1807
variable_sites: 103
pi: 0.0602
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	ATATGTAGGAGGTGGTCGCA	50.0	50.0	58.2	57.9	1969-1975 bp	1.0	86.2
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	TATGTAGGAGGTGGTCGCAT	50.0	50.0	58.2	57.9	1968-1974 bp	1.0	86.2
trnN-GUU-ndhF_p3	AATCAGTTAACAGCCGACCG	ATATGTAGGAGGTGGTCGCA	50.0	50.0	58.3	57.9	1950-1956 bp	1.0	85.3
trnN-GUU-ndhF_p4	AATCAGTTAACAGCCGACCG	TATGTAGGAGGTGGTCGCAT	50.0	50.0	58.3	57.9	1949-1955 bp	1.0	85.3
trnN-GUU-ndhF_p5	CCCCAAGTAGGATTCGAACC	TATGTAGGAGGTGGTCGCAT	55.0	50.0	57.7	57.9	1976-1982 bp	1.0	84.8