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rps19-rpl12

LSC · 86459-86525 · 67 bp

Back to Bruinsmia

Pi 0.164

MarkerSeek score 72.50

Primer available yes

Label rps19-rpl12

Feature Metadata

label_name: rps19-rpl12
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 11
pi: 0.164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl12_p1	TAATGGTAGATGCCCGGGAC	TTGATTCTTCGTCGCCGTAG	55.0	50.0	58.9	58.1	215-220 bp	1.0	76.1
rps19-rpl12_p2	TAATGGTAGATGCCCGGGAC	CACAACCCCTTGGGGTTATC	55.0	55.0	58.9	58.2	282-287 bp	1.0	74.6
rps19-rpl12_p3	TAATGGTAGATGCCCGGGAC	ATTTGATTCTTCGTCGCCGT	55.0	45.0	58.9	58.3	217-222 bp	1.0	74.6
rps19-rpl12_p4	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	59.2	58.1	204-209 bp	1.0	74.6
rps19-rpl12_p5	TAATGGTAGATGCCCGGGAC	TTGGGGTTATCCTGCACTTG	55.0	50.0	58.9	57.8	273-278 bp	1.0	74.2