Hotspot

petA-psbJ

LSC · 65190-66234 · 1045 bp

Back to Bruinsmia

Pi 0.0117

MarkerSeek score 68.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1045
variable_sites: 11
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1089-1188 bp	1.0	80.4
petA-psbJ_p2	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1090-1189 bp	1.0	80.4
petA-psbJ_p3	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	1084-1183 bp	1.0	77.6
petA-psbJ_p4	CGTGTTCAAGGCCTTTTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	1083-1182 bp	1.0	77.2
petA-psbJ_p5	CCATTACGTGTTCAAGGCCT	TGGTTCATATTCCGGATTGGG	50.0	47.6	58.2	57.8	1097-1196 bp	1.0	73.6