Hotspot

atpB-rbcL

LSC · 55365-55807 · 443 bp

Back to Bruinsmia

Pi 0.0442

MarkerSeek score 83.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 19
pi: 0.0442
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AACGATAAGCCCCGGTTTTT	CACCAGCTTTGAATCCAACAC	45.0	47.6	58.1	58.0	564-912 bp	1.0	74.6
atpB-rbcL_p2	AACGATAAGCCCCGGTTTTT	ACCAGCTTTGAATCCAACACT	45.0	42.9	58.1	58.1	563-911 bp	1.0	74.4
atpB-rbcL_p3	GGCGGAAAGGCTACATCTAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.8	58.0	602-950 bp	1.0	73.9
atpB-rbcL_p4	GGCGGAAAGGCTACATCTAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.8	58.1	601-949 bp	1.0	73.6
atpB-rbcL_p5	ATTTGAACGATAAGCCCCGG	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.3	58.0	569-917 bp	1.0	72.7