primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	atpH-atpF	1	GGCTGGTTGTAGCATTAGCA	CCAGTAGCCCAAAGAAACGA	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.428719	20922	21288	456-471	1.000000	79.3
atpH-atpF_p2	atpH-atpF	2	TGGGCTGGTTGTAGCATTAG	CCAGTAGCCCAAAGAAACGA	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.597280	20922	21288	458-473	1.000000	78.0
atpH-atpF_p3	atpH-atpF	3	GGCTGGTTGTAGCATTAGCA	TATTAAGCCCGAAACTCCCG	20	20	50.0	50.0	58.2	57.4	0.0	0.0	0.857438	20922	21288	484-499	1.000000	75.7
atpH-atpF_p4	atpH-atpF	4	CGGAGGGAAAAATACGAGGT	CCAGTAGCCCAAAGAAACGA	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.864762	20922	21288	522-537	1.000000	75.5
atpH-atpF_p5	atpH-atpF	5	GCGGAGGGAAAAATACGAGG	CCAGTAGCCCAAAGAAACGA	20	20	55.0	50.0	58.7	57.8	0.0	0.0	0.882185	20922	21288	523-538	1.000000	75.4
trnS-UGA_p1	trnS-UGA	1	TTTTCTGGCTTGGCTAGGTG	TCGACCTCTAGATTCACACCT	20	21	50.0	47.6	58.1	57.6	3.3	0.0	1.457946	35583	35671	298-298	1.000000	69.4
trnS-UGA_p2	trnS-UGA	2	GATAGCCGAGCCATTTTCCT	TCGACCTCTAGATTCACACCT	20	21	50.0	47.6	57.7	57.6	0.0	0.0	1.630069	35583	35671	277-277	1.000000	68.1
trnS-UGA_p3	trnS-UGA	3	CCGGTCCGGTAAAAACCAAT	TCGACCTCTAGATTCACACCT	20	21	50.0	47.6	58.5	57.6	23.0	0.0	1.830706	35583	35671	245-245	1.000000	66.7
trnS-UGA_p4	trnS-UGA	4	TAGCCGAGCCATTTTCCTTT	TCGACCTCTAGATTCACACCT	20	21	45.0	47.6	57.5	57.6	0.0	0.0	1.875053	35583	35671	275-275	1.000000	66.2
trnS-UGA_p5	trnS-UGA	5	TTTTCTGGCTTGGCTAGGTG	CGATAGGGGTGGTATTCACT	20	20	50.0	50.0	58.1	56.1	3.3	0.0	1.978974	35583	35671	264-264	1.000000	65.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	46924	47713	840-866	1.000000	84.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	46924	47713	848-874	1.000000	83.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	46924	47713	872-898	1.000000	83.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	46924	47713	880-906	1.000000	82.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	46924	47713	854-880	1.000000	82.5
trnF-GAA_p1	trnF-GAA	1	ACGACCCCAATCTGTATCCT	TTGTCTTATGGTACGCGTGG	20	20	50.0	50.0	57.8	58.0	0.0	24.6	0.177199	48689	48761	1345-1355	1.000000	80.8
trnF-GAA_p2	trnF-GAA	2	ACGACCCCAATCTGTATCCT	AGTCATGTGGGAGGGAATGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.205098	48689	48761	1170-1179	1.000000	80.7
trnF-GAA_p3	trnF-GAA	3	ACGACCCCAATCTGTATCCT	AGAAAGTCATGTGGGAGGGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.221040	48689	48761	1174-1183	1.000000	80.5
trnF-GAA_p4	trnF-GAA	4	TGACTGTGTCGGTAGAGGAA	TTGTCTTATGGTACGCGTGG	20	20	50.0	50.0	57.7	58.0	0.0	24.6	0.282491	48689	48761	1445-1455	1.000000	79.9
trnF-GAA_p5	trnF-GAA	5	ACGACCCCAATCTGTATCCT	GCGTGGTCCAACAACAAAAA	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.178578	48689	48761	1341-1341	0.500000	58.6
atpB-rbcL_p1	atpB-rbcL	1	AACGATAAGCCCCGGTTTTT	CACCAGCTTTGAATCCAACAC	20	21	45.0	47.6	58.1	58.0	0.0	0.0	1.103487	55365	55807	564-912	1.000000	74.6
atpB-rbcL_p2	atpB-rbcL	2	AACGATAAGCCCCGGTTTTT	ACCAGCTTTGAATCCAACACT	20	21	45.0	42.9	58.1	58.1	0.0	0.0	1.136775	55365	55807	563-911	1.000000	74.4
atpB-rbcL_p3	atpB-rbcL	3	GGCGGAAAGGCTACATCTAG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	57.8	58.0	0.0	0.0	1.173608	55365	55807	602-950	1.000000	73.9
atpB-rbcL_p4	atpB-rbcL	4	GGCGGAAAGGCTACATCTAG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	57.8	58.1	0.0	0.0	1.206896	55365	55807	601-949	1.000000	73.6
atpB-rbcL_p5	atpB-rbcL	5	ATTTGAACGATAAGCCCCGG	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.3	58.0	0.0	0.0	1.341713	55365	55807	569-917	1.000000	72.7
rbcL-accD_p1	rbcL-accD	1	GAGGCTAGCAAATGGAGTCC	AGCATACTCGATCAAACGGG	20	20	55.0	50.0	58.0	57.8	18.2	0.0	0.264652	57236	59846	2757-2914	1.000000	89.8
rbcL-accD_p2	rbcL-accD	2	AATGAGGGACGTGATCTTGC	AGCATACTCGATCAAACGGG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.254906	57236	59846	2802-2959	1.000000	89.7
rbcL-accD_p3	rbcL-accD	3	GCTGCTTGTGAGGTATGGAA	AGCATACTCGATCAAACGGG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.338929	57236	59846	2727-2884	1.000000	89.2
rbcL-accD_p4	rbcL-accD	4	AATGAGGGACGTGATCTTGC	ATAACCCCAATTGCTACGGG	20	20	50.0	50.0	58.0	57.6	0.0	14.1	0.388160	57236	59846	2729-2886	1.000000	88.9
rbcL-accD_p5	rbcL-accD	5	TGATCTTGCTCGTGAGGGTA	AGCATACTCGATCAAACGGG	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.377740	57236	59846	2791-2948	1.000000	88.8
petA-psbJ_p1	petA-psbJ	1	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	0.0	4.1	0.543756	65190	66234	1089-1188	1.000000	80.4
petA-psbJ_p2	petA-psbJ	2	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	0.0	4.1	0.548898	65190	66234	1090-1189	1.000000	80.4
petA-psbJ_p3	petA-psbJ	3	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.5	57.6	17.6	4.1	0.894921	65190	66234	1084-1183	1.000000	77.6
petA-psbJ_p4	petA-psbJ	4	CGTGTTCAAGGCCTTTTGTT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.4	57.6	17.6	4.1	0.942422	65190	66234	1083-1182	1.000000	77.2
petA-psbJ_p5	petA-psbJ	5	CCATTACGTGTTCAAGGCCT	TGGTTCATATTCCGGATTGGG	20	21	50.0	47.6	58.2	57.8	0.0	4.1	1.400956	65190	66234	1097-1196	1.000000	73.6
rps12-psbB_p1	rps12-psbB	1	GCGGGAGATTTCGTGACATT	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.6	58.2	0.0	0.0	0.867668	72485	74716	2231-2320	1.000000	81.1
rps12-psbB_p2	rps12-psbB	2	GAAGAGCGGGAGATTTCGTG	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	58.7	58.2	0.0	0.0	0.955393	72485	74716	2236-2325	1.000000	80.4
rps12-psbB_p3	rps12-psbB	3	GCGGGAGATTTCGTGACATT	ATACAGAGCCATTGAACCGG	20	20	50.0	50.0	58.6	57.7	0.0	0.0	0.962438	72485	74716	2331-2420	1.000000	80.2
rps12-psbB_p4	rps12-psbB	4	GTACATGTTCCTCGACGCTG	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	58.7	58.2	2.3	0.0	0.968127	72485	74716	2267-2356	1.000000	80.2
rps12-psbB_p5	rps12-psbB	5	GCGGGAGATTTCGTGACATT	AGCAACCAAAGCTGTATGCA	20	20	50.0	45.0	58.6	58.4	0.0	10.2	1.010721	72485	74716	2304-2393	1.000000	79.8
infA-rps8_p1	infA-rps8	1	CGAACCCGGAACATACCATT	GAACTCGAGTTTTTGGTGCG	20	20	50.0	50.0	58.0	58.0	0.7	30.2	0.051398	82400	82520	913-924	1.000000	83.8
infA-rps8_p2	infA-rps8	2	AGAAACCACCCGACCTCTTA	GAACTCGAGTTTTTGGTGCG	20	20	50.0	50.0	58.0	58.0	0.0	30.2	0.038524	82400	82520	1571-1612	1.000000	82.6
infA-rps8_p3	infA-rps8	3	CGTGAACCAATACGTCCACT	GAACTCGAGTTTTTGGTGCG	20	20	50.0	50.0	57.9	58.0	0.0	30.2	0.093264	82400	82520	1471-1512	1.000000	82.3
infA-rps8_p4	infA-rps8	4	CGAACCCGGAACATACCATT	GAAGGAATCGGCGGAGAAAT	20	20	50.0	50.0	58.0	58.0	0.7	7.2	0.077753	82400	82520	237-278	1.000000	82.2
infA-rps8_p5	infA-rps8	5	AGAAACCACCCGACCTCTTA	GAAGGAATCGGCGGAGAAAT	20	20	50.0	50.0	58.0	58.0	0.0	7.2	0.064880	82400	82520	895-966	1.000000	81.0
rps19-rpl12_p1	rps19-rpl12	1	TAATGGTAGATGCCCGGGAC	TTGATTCTTCGTCGCCGTAG	20	20	55.0	50.0	58.9	58.1	29.3	0.0	1.042432	86459	86525	215-220	1.000000	76.1
rps19-rpl12_p2	rps19-rpl12	2	TAATGGTAGATGCCCGGGAC	CACAACCCCTTGGGGTTATC	20	20	55.0	55.0	58.9	58.2	29.3	35.0	1.099248	86459	86525	282-287	1.000000	74.6
rps19-rpl12_p3	rps19-rpl12	3	TAATGGTAGATGCCCGGGAC	ATTTGATTCTTCGTCGCCGT	20	20	55.0	45.0	58.9	58.3	29.3	0.0	1.224565	86459	86525	217-222	1.000000	74.6
rps19-rpl12_p4	rps19-rpl12	4	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	20	20	55.0	50.0	59.2	58.1	29.3	0.0	1.260580	86459	86525	204-209	1.000000	74.6
rps19-rpl12_p5	rps19-rpl12	5	TAATGGTAGATGCCCGGGAC	TTGGGGTTATCCTGCACTTG	20	20	55.0	50.0	58.9	57.8	29.3	0.0	1.160952	86459	86525	273-278	1.000000	74.2
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	AGGATTCGAACCTACGACCA	ATATGTAGGAGGTGGTCGCA	20	20	50.0	50.0	58.2	57.9	15.0	0.0	0.238772	111275	113081	1969-1975	1.000000	86.2
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	AGGATTCGAACCTACGACCA	TATGTAGGAGGTGGTCGCAT	20	20	50.0	50.0	58.2	57.9	15.0	0.0	0.238772	111275	113081	1968-1974	1.000000	86.2
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	AATCAGTTAACAGCCGACCG	ATATGTAGGAGGTGGTCGCA	20	20	50.0	50.0	58.3	57.9	8.6	0.0	0.360129	111275	113081	1950-1956	1.000000	85.3
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	AATCAGTTAACAGCCGACCG	TATGTAGGAGGTGGTCGCAT	20	20	50.0	50.0	58.3	57.9	8.6	0.0	0.360129	111275	113081	1949-1955	1.000000	85.3
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	CCCCAAGTAGGATTCGAACC	TATGTAGGAGGTGGTCGCAT	20	20	55.0	50.0	57.7	57.9	0.0	0.0	0.409340	111275	113081	1976-1982	1.000000	84.8
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	CCCGCTTTCAACCGAATCTA	CAACCACTCGGATTGGAGAG	20	20	50.0	55.0	58.0	58.0	0.0	19.9	0.034278	117358	117469	1044-1045	1.000000	82.0
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	CCCGCTTTCAACCGAATCTA	ACCCGAAGTAGTAAAGCCCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.070151	117358	117469	984-985	1.000000	81.9
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	AACAAGCCGCTATGGTGAAA	CAACCACTCGGATTGGAGAG	20	20	45.0	55.0	58.1	58.0	0.0	19.9	0.111449	117358	117469	605-607	1.000000	81.4
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	TTATGCAGTGCGAATTCGGA	CAACCACTCGGATTGGAGAG	20	20	45.0	55.0	58.0	58.0	27.8	19.9	0.032874	117358	117469	1256-1256	0.500000	59.7
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	TTATGCAGTGCGAATTCGGA	ACCCGAAGTAGTAAAGCCCT	20	20	45.0	50.0	58.0	58.1	27.8	0.0	0.068747	117358	117469	1196-1196	0.500000	59.5
ndhI-ndhA_p1	ndhI-ndhA	1	TGCAGCTCGTACTGTTTGTT	GATGGACTTTACCGAGGCTG	20	20	45.0	55.0	58.1	58.1	8.8	0.0	0.110571	122743	122842	276-276	1.000000	81.1
ndhI-ndhA_p2	ndhI-ndhA	2	TTGCAGCTCGTACTGTTTGT	GATGGACTTTACCGAGGCTG	20	20	45.0	55.0	58.1	58.1	8.8	0.0	0.110571	122743	122842	277-277	1.000000	81.1
ndhI-ndhA_p3	ndhI-ndhA	3	TGCAGCTCGTACTGTTTGTT	TTACCGAGGCTGAGAATGGA	20	20	45.0	50.0	58.1	58.1	8.8	0.0	0.191255	122743	122842	268-268	1.000000	80.6
ndhI-ndhA_p4	ndhI-ndhA	4	TGCAGCTCGTACTGTTTGTT	AGATGGACTTTACCGAGGCT	20	20	45.0	50.0	58.1	58.1	8.8	0.0	0.184533	122743	122842	277-277	1.000000	80.5
ndhI-ndhA_p5	ndhI-ndhA	5	TTGCAGCTCGTACTGTTTGT	AGATGGACTTTACCGAGGCT	20	20	45.0	50.0	58.1	58.1	8.8	0.0	0.184533	122743	122842	278-278	1.000000	80.5
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	ATGCTCTGCTGGCATTACTC	AAGATCCTTCTTCTGCCCCT	20	20	50.0	50.0	58.0	58.0	11.8	0.0	0.057086	130911	131030	1136-1163	1.000000	88.0
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	ATGCTCTGCTGGCATTACTC	AGATCCTTCTTCTGCCCCTT	20	20	50.0	50.0	58.0	58.0	11.8	0.0	0.057086	130911	131030	1135-1162	1.000000	88.0
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	ATGCTCTGCTGGCATTACTC	ACGAAGGGGACTAAACAGGA	20	20	50.0	50.0	58.0	58.0	11.8	0.0	0.055255	130911	131030	1176-1203	1.000000	87.8
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	CTTTGGGCTCGGATAGAACC	ACGAAGGGGACTAAACAGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.064880	130911	131030	1373-1424	1.000000	86.8
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	GGGCTCGGATAGAACCTTTG	ACGAAGGGGACTAAACAGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.064880	130911	131030	1369-1420	1.000000	86.8