Hotspot

ndhB-trnL-CAA

IRa · 154125-154778 · 654 bp

Back to Bruguiera

Pi 0.00367
MarkerSeek score 52.00
Primer available yes
Label ndhB-trnL-CAA

Feature Metadata

label_name
ndhB-trnL-CAA
feature_type
igs
region
IRa
length_bp
654
variable_sites
5
pi
0.00367
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnL-CAA_p1 CCAGGAATATCAATGAACCCGA GTCTCGCGTGTCTACCATTT 45.5 50.0 57.9 58.0 741-770 bp 1.0 63.8
ndhB-trnL-CAA_p2 CCAGGAATATCAATGAACCCGA CTCGCGTGTCTACCATTTCA 45.5 50.0 57.9 58.0 739-768 bp 1.0 63.8
ndhB-trnL-CAA_p3 CCAGGAATATCAATGAACCCGA TACCATTTCACCACCAAGGC 45.5 50.0 57.9 58.1 729-758 bp 1.0 63.2
ndhB-trnL-CAA_p4 ACCAGGAATATCAATGAACCCG GTCTCGCGTGTCTACCATTT 45.5 50.0 58.2 58.0 742-771 bp 1.0 63.0
ndhB-trnL-CAA_p5 ACCAGGAATATCAATGAACCCG CTCGCGTGTCTACCATTTCA 45.5 50.0 58.2 58.0 740-769 bp 1.0 63.0