Hotspot

trnL-CAA-ndhB

IRb · 101258-101911 · 654 bp

Back to Bruguiera

Pi 0.00367

MarkerSeek score 52.00

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 654
variable_sites: 5
pi: 0.00367
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.0	57.9	741-770 bp	1.0	63.8
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.0	57.9	739-768 bp	1.0	63.8
trnL-CAA-ndhB_p3	TACCATTTCACCACCAAGGC	CCAGGAATATCAATGAACCCGA	50.0	45.5	58.1	57.9	729-758 bp	1.0	63.2
trnL-CAA-ndhB_p4	GTCTCGCGTGTCTACCATTT	ACCAGGAATATCAATGAACCCG	50.0	45.5	58.0	58.2	742-771 bp	1.0	63.0
trnL-CAA-ndhB_p5	CTCGCGTGTCTACCATTTCA	ACCAGGAATATCAATGAACCCG	50.0	45.5	58.0	58.2	740-769 bp	1.0	63.0